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Peptide Dosing
& Reconstitution

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Featured Protocols

Most Researched Peptide Guides

Each protocol includes reconstitution math, BAC water volumes, syringe-specific injection volumes, and a full citation chain.

Blend

BPC-157 + TB-500 20mg

● 250–500 mcg/day● SubQ

High-dose recovery blend combining two of the most researched tissue-support peptides. Full titration schedule and reconstitution math included.

View Full Protocol
GLP-1

Semaglutide Reconstitution

● 0.25–2.4 mg/week● Weekly SubQ

Weekly dose escalation from 0.25mg to 2.4mg. Injection volume by syringe type at each escalation stage. Common errors documented.

View Full Protocol
GH Secretagogue

CJC-1295 + Ipamorelin 10mg

● 100/100 mcg● Pre-sleep

GHRH + GHRP synergy explained. Reconstitution for 5mg, 10mg, and 20mg blend vials with full dosing schedule and timing guide.

View Full Protocol
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All reconstitution calculations are triple-checked. Our open calculator lets anyone verify independently. Errors, if found, are corrected publicly.
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Browse 180+ peptide protocols. Each lists the research dose range, administration route, and cycle length from peer-reviewed sources.
02
Calculate Your Volumes
Enter your vial size and target dose. Get exact mL and syringe unit values for U-100, U-50, and U-20 syringes instantly.
03
Verify Your COA
Use our 12-point COA checklist to authenticate your Certificate of Analysis before reconstitution. Spot fakes in under two minutes.
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COA Verification
12 red flags documented. Real vs. fake certificates compared side-by-side with expert annotation.
Community Feed
Live comments under every protocol. Real researcher input, corrections, and experiences.
4,872 Reports
Aggregate outcome data spanning 94 unique peptides and blends from the research community.
Dose Calculator
Exact volumes for U-100, U-50, and U-20 syringes. Instant results, no login required.
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The Most Complete Peptide Research Resource

Join thousands of researchers using verified protocols. Free forever, or upgrade to Pro for the full PDF library and monthly digest.

Dosage Protocols

Research Peptide Protocols

Each protocol includes reconstitution math, BAC water volumes, injection volumes per syringe type, and full citation chain. Comments open under each one.

Blend

BPC-157 + TB-500 20mg

● 250–500 mcg/day● SubQ

High-dose recovery blend combining two of the most researched tissue-support peptides. Titration schedule and full reconstitution math included.

View Full Protocol
Researcher J.T.
Running at 250mcg/day SubQ. Week 3 — significant reduction in knee inflammation. Reconstituted with 4mL BAC water. 5 units on U-100.
2025-03-18
Researcher A.M.
Let the vial sit 10 min after adding BAC water — dissolves fully without agitation. Confirmed the math matches the calculator exactly.
2025-02-27
Researcher D.K.
Is the 20mg blend equivalent to running 10mg BPC + 10mg TB-500 separately? Asking because I only have individual vials.
2025-02-14
GLP-1

Retatrutide 20mg

● 0.5–12 mg/week● SubQ

Triple-agonist GLP-1/GIP/glucagon receptor peptide. Full dosing ramp and weekly dose escalation from Phase 2 trial data.

View Full Protocol
Researcher P.W.
Starting at 0.5mg/week for first 4 weeks. Nausea minimal compared to semaglutide. Appetite suppression noticeable by day 3.
2025-03-20
Researcher S.L.
20mg vial + 4mL BAC = 5mg/mL. 0.5mg dose = 10 units on U-100. Confirmed with the calculator.
2025-03-05
GH Secretagogue

CJC-1295 + Ipamorelin 10mg

● 100/100 mcg● Pre-sleep

GHRH + GHRP synergy explained. Reconstitution for 5mg, 10mg, and 20mg blend vials with full dosing schedule.

View Full Protocol
Researcher M.B.
Pre-sleep timing is key. 100/100mcg 30 min before bed. Sleep improvement was the first noticeable effect, around week 2.
2025-03-15
Researcher T.R.
10mg blend + 2mL BAC = 2.5mg/mL each. 4 units on U-100 = 100mcg per peptide. Calculator confirmed.
2025-03-01
GLP-1

Semaglutide Reconstitution

● 0.25–2.4 mg/week● Weekly SubQ

Weekly dose escalation from 0.25mg to 2.4mg. Injection volume by syringe type at each escalation stage. Common errors documented.

View Full Protocol
Researcher C.F.
Common mistake: 0.5mL BAC for 5mg vial = 10mg/mL — too concentrated. Use 2.5mL minimum for accurate dosing.
2025-03-22
Single

TB-500 (Thymosin Beta-4)

● 2.5 mg ×2/week● SubQ / IM

Reconstitution for 5mg and 10mg TB-500 vials. Dose range, injection frequency, and storage per clinical literature.

View Full Protocol
Researcher R.H.
2.5mg twice weekly loading, then 1.25mg/week maintenance. 1mL BAC water: 50 units = 2.5mg on U-100.
2025-02-28
Nootropic

Semax + Selank Stack

● 300–600 mcg/day● Intranasal

Intranasal administration guide. Concentration ratios, drops-per-dose conversion, and storage for combined ACTH/anxiolytic stack.

View Full Protocol
Researcher N.V.
600mcg/day caused nasal irritation by day 4. Dropped to 300mcg and resolved. Start lower than the standard protocol suggests.
2025-03-10
Single

BPC-157 5mg & 10mg

● 200–500 mcg/day● SubQ or oral

Complete standalone BPC-157 protocol. Subcutaneous and oral administration routes. Reconstitution tables for 5mg and 10mg vials.

View Full Protocol
Researcher L.P.
5mg + 2.5mL BAC = 2mg/mL. 250mcg = 12.5 units on U-100. No injection site reactions after 4 weeks.
2025-03-25
GH Secretagogue

GHRP-6 / GHRP-2 Protocol

● 100–300 mcg● 3× daily SubQ

First-generation GHRP comparison guide. Hunger profile, dosing windows, and combination protocol with GHRH analogues.

View Full Protocol
Researcher K.S.
GHRP-6 hunger effect is significant at 300mcg. Time around meals. GHRP-2 is cleaner at equivalent doses.
2025-03-08
Blend

Tesamorelin + Ipamorelin

● 1–2 mg/day● SubQ abdomen

FDA-approved GHRH + GHRP combination. Reconstitution, abdominal fat reduction data from clinical trials, and cycle duration guidance.

View Full Protocol
Researcher B.F.
Tesamorelin 2mg + Ipamorelin 200mcg pre-sleep. Week 8: visible reduction in visceral fat. IGF-1 bloodwork confirmed GH pulse.
2025-02-20
Authentication & Fraud Detection

Real vs. Fake Peptide COA:
The Complete Verification Guide

A Certificate of Analysis is the only third-party evidence of what is actually in a vial. Learn to distinguish legitimate lab reports from fabricated documents in under two minutes.

12 Red Flags Documented 8 Verification Checkpoints Fraud Examples Included
Side-by-Side Analysis

Real COA vs. Fabricated COA

Legitimate COA — What to Look For
ANALYTICAL LABORATORIES ACCREDITED LLC
ISO/IEC 17025:2017 Accredited  |  A2LA #4821.01  |  CLIA ID: 99D1234567
123 Research Parkway, Suite 400, Boston MA 02115  |  (617) 555-0182
Report Number:RPT-2024-09847-A
Date Sample Received:2024-08-10
Date of Analysis:2024-08-14
Product:BPC-157 Research Peptide
Lot / Batch:LOT-BPC-240810-04
Accreditation verifiable on A2LA public registry. Two dates (received + analyzed). Unique report number. Physical address present.
HPLC Purity:98.7%
MS Molecular Weight:1419.53 Da (Exp: 1419.54 Da)
Endotoxin (LAL):<0.10 EU/mg
Analyst:Dr. M. Patterson (signed)
QA Sign-off:J. Reyes – QA Manager
Purity 98.7% — not round. MS confirms MW within 0.01 Da. Endotoxin tested. Chromatogram attached. Dual signature.
Fabricated COA — Red Flags Annotated
PREMIUM QUALITY LABS INTERNATIONAL
Quality Testing Services  |  info@premiumqualitylabs.com
No physical address listed
Report Number:COA-001
Date of Analysis:January 2024
Product:BPC-157 5mg
Lot / Batch:BPC157
No ISO accreditation. Domain registered 3 weeks before COA. “COA-001” not unique. No address. Month-only date = no workflow.
Purity:99.00%
Molecular Weight:Confirmed
Endotoxin:Not tested
Analyst:Not signed
Exactly 99.00% never occurs in real HPLC. “Confirmed” without Da value. No chromatogram. No endotoxin test. No signature.
Fraud Detection Checklist

12 Red Flags in Peptide COAs

RED FLAG 01
No Accreditation Number
Legitimate labs carry ISO/IEC 17025 accreditation with a publicly searchable number. No number = no verification possible.
RED FLAG 02
Suspiciously Round Purity
HPLC produces decimals like 97.3% or 98.6%. Exactly 99.00% or 98.00% is a strong indicator of a fabricated result.
RED FLAG 03
No HPLC Chromatogram
A real HPLC result includes the chromatogram trace showing peaks and retention times. Purity without its source chart is unverifiable.
RED FLAG 04
“Confirmed” Not a Da Value
Mass spectrometry produces a specific dalton value. “Confirmed” without the Da number means no MS was performed.
RED FLAG 05
Unverifiable Lab Name
Search the lab name + city in regulatory databases. Generic names with no physical address are high risk.
RED FLAG 06
Mismatched Fonts / Formatting
Legitimate documents use consistent typography. Font changes or misaligned tables indicate assembly from multiple sources.
RED FLAG 07
Lot Number Mismatch
The lot number on the COA must exactly match the lot on your vial. Any mismatch means the COA was not issued for your product.
RED FLAG 08
No Date of Sample Receipt
A real COA shows when the sample arrived and when testing was completed — two dates. Month-only = no real testing workflow.
RED FLAG 09
No Analyst Signature
Accredited lab reports require sign-off by the testing analyst and a QA officer. Unsigned = no chain of custody.
RED FLAG 10
One COA for Multiple Products
Some vendors reuse one COA across different peptides. Cross-check the report date and lot number against other products sold.
RED FLAG 11
Lab Domain Registered Recently
Check the lab domain via WHOIS. A “testing laboratory” whose domain was registered within 12 months is a major fraud indicator.
RED FLAG 12
Missing Endotoxin Data
Injectable-grade compounds should include LAL endotoxin testing. Absence for injectable peptides is a quality control gap at minimum.

Download the COA Verification Checklist

Pro members get the printable 12-point checklist PDF. Keep it on hand every time a new vial arrives.

Researcher Experiences

Real Results. Real Failures.
Real Data.

Community-built database of researcher experiences. Every submission anonymized before publishing. Share your protocol — positive, negative, or inconclusive.

4,872Total Submissions
94Peptides Documented
74%Positive Outcomes
18%Mixed Outcomes
8%Negative / No Effect
Researcher M.K.·2025-03-12
Positive

BPC-157 + TB-500 blend — tendon recovery after 6-week protocol

BPC-157TB-500

Running BPC-157 + TB-500 20mg blend at 250mcg/day SubQ for 6 weeks. Reconstituted with 4mL BAC water. Used the calculator — 5 units on U-100.

Week 2: Noticeably reduced acute inflammation. Ultrasound at week 4 showed improved tendon architecture. No injection site reactions beyond mild redness at first injection. Did not use NSAIDs during the protocol.

Duration:6 weeks
Dose:250 mcg/day
Researcher D.T.·2025-02-28
Negative

Semax intranasal — no cognitive outcome, significant nasal irritation

SemaxIntranasal

3-week protocol: Semax 600mcg/day intranasal. Persistent nasal irritation began day 4. Discontinued at day 15.

No subjective cognitive enhancement. Mucosa dryness and mild burning. COA showed 98.3% purity — likely not a quality issue. Would try lower dose (200mcg/day) or subcutaneous route. Reporting as failure so others know.

Duration:15 days
Dose:600 mcg/day
Researcher A.R.·2025-03-01
Mixed

Semaglutide reconstitution error — corrected with calculator

SemaglutideError Report

Documenting a reconstitution error: used 0.5mL BAC water on a 5mg vial (10mg/mL) — dose volume too small to measure on U-100.

Corrected to 2.5mL BAC (2mg/mL). 0.25mg dose = 12.5 units on U-100 — measurable. Appetite suppression noticeable by day 4 after correction. Error was entirely reconstitution math.

Dose:0.25 mg/week
Researcher J.W.·2025-03-18
Positive

CJC-1295 + Ipamorelin — 12-week GH pulse protocol with bloodwork

CJC-1295Ipamorelin

100mcg/100mcg pre-sleep SubQ for 12 weeks. Reconstituted 10mg blend with 2mL BAC — 4 units on U-100. IGF-1 bloodwork at weeks 6 and 12.

IGF-1 rose from 182 ng/mL to 234 ng/mL at week 6, stabilized at 228 ng/mL at week 12. Sleep quality markedly improved by week 2. No desensitization on 5-on/2-off schedule.

Duration:12 weeks
Dose:100/100 mcg nightly
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Peptide Reconstitution Calculator

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Reconstitution Tips

  • Inject BAC water slowly down the inside wall of the vial — never directly onto the lyophilized powder.
  • Do not shake. Swirl gently or let stand 10 minutes. Vigorous agitation degrades peptide bonds.
  • Store reconstituted peptides at 2–8°C. Use within 28 days for most peptides.
  • Use a 27–29 gauge needle for SubQ injection. Rotate injection sites each time.
  • For very small doses, dilute further with additional BAC water to improve measurement accuracy.